Journal: Materials Today Bio

Article Title: Collagen-elastin dermal scaffolds enhance tissue regeneration and reduce scarring in preclinical models

doi: 10.1016/j.mtbio.2025.102239

Figure Lengend Snippet: Results from cell culture experiments of primary human dermal fibroblasts (adult, eschar, and fetal) in the presence of scaffolds (day 14, n = 3). A) Cellular localization within scaffolds analyzed through H&E staining. Scale bar is 50 μm. B-D) Comparative gene expression levels of COL1A1 , ELN , and ACTA2 across three fibroblast types, normalized to the mean expression of adult fibroblasts cultured on COL scaffolds. Differences in gene expression levels between scaffold types and fibroblast types were tested using two-way ANOVA, followed by Tukey's multiple comparisons test (α = 0.05). Only statistically significant differences between scaffolds within the same fibroblast type are shown, while full pairwise comparisons are provided in ) Relative expression of α-SMA based on intensities of the bands on Western blot, normalized to COL scaffolds for the same type of fibroblasts. Individual donors are represented by different symbols as ● donor 1/4/7, ■ donor 2/5/8, and ▲ donor 3/6/9. Error bars represent standard deviation; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. COL = type I collagen scaffold, COL:ELN-A = type I collagen scaffold supplemented with ELN-A, COL:ELN-B = type I collagen scaffold supplemented with ELN-B, COL1A1 = alpha-1 type I collagen, ELN = elastin, ACTA2 = smooth muscle actin alpha 2, α-SMA = α-smooth muscle actin.

Article Snippet: Visualization of α-SMA was performed similarly, using a mouse monoclonal anti-α-SMA antibody (A2547, 1:200) and a biotinylated goat anti-mouse IgG (Vector Laboratories, BA-9200, 1:200).

Techniques: Cell Culture, Staining, Gene Expression, Expressing, Western Blot, Standard Deviation

Journal: Materials Today Bio

Article Title: Collagen-elastin dermal scaffolds enhance tissue regeneration and reduce scarring in preclinical models

doi: 10.1016/j.mtbio.2025.102239

Figure Lengend Snippet: Microscopic examination of healthy and healed rat skin at day 56. A) Representative sections stained for α-SMA, in brown. Collagen fibers visualized with SHG using multiphoton microscopy. Sections stained for elastin, in brown. Scale bars are 50 μm. B) Section stained for α-SMA, presenting an area with strong positive staining, co-localizing with residual scaffold fragments (yellow arrow), surrounded by foreign body giant cells (grey arrow) (magnified area, H&E). α-SMA staining also highlights neovascularization (magnified area, red arrow). Black scale bar is 200 μm, grey and white scale bars are 20 μm. COL = type I collagen scaffold, COL:ELN-A = type I collagen scaffold supplemented with ELN-A, COL:ELN-B = type I collagen scaffold supplemented with ELN-B, α-SMA = α-smooth muscle actin, SHG = second harmonic generation, ELN = elastin, H&E = hematoxylin and eosin. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Visualization of α-SMA was performed similarly, using a mouse monoclonal anti-α-SMA antibody (A2547, 1:200) and a biotinylated goat anti-mouse IgG (Vector Laboratories, BA-9200, 1:200).

Techniques: Staining, Microscopy